X-ray diffraction
2Å resolution

Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from Ralstonia metallidurans complexed with 4-chloro-3-hydroxyanthranilic acid and NO


Function and Biology Details

Reaction catalysed:
3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-hydroxyanthranilate 3,4-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.06 KDa
Source organism: Cupriavidus metallidurans
Expression system: Escherichia coli
  • Canonical: Q1LCS4 (Residues: 1-174; Coverage: 100%)
Gene names: Rmet_5193, nbaC
Sequence domains: 3-hydroxyanthranilic acid dioxygenase
Structure domains: Jelly Rolls

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P6522
Unit cell:
a: 57.9Å b: 57.9Å c: 230.6Å
α: 90° β: 90° γ: 120°
R R work R free
0.244 0.244 0.291
Expression system: Escherichia coli