PDBe 1yf2

X-ray diffraction
2.4Å resolution

Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-1 restriction enzyme MjaXIP specificity protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 425 amino acids
Theoretical weight: 48.64 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57594 (Residues: 1-425; Coverage: 100%)
Gene name: MJ0130
Sequence domains: Type I restriction modification DNA specificity domain
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 71.972Å b: 94.222Å c: 103.52Å
α: 90° β: 95.01° γ: 90°
R-values:
R R work R free
0.236 0.236 0.279
Expression system: Escherichia coli BL21(DE3)