› 1ye2

X-ray diffraction
1.8Å resolution

T-To-T(High) quaternary transitions in human hemoglobin: betaY35F oxy (2MM IHP, 20% PEG) (1 test set)

Released:
DOI: 10.2210/pdb1ye2/pdb
PDB 1ye2 coloured by chain and viewed from the front.
PDB 1ye2 coloured by chain and viewed from the side.
PDB 1ye2 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Crystallographic evidence for a new ensemble of ligand-induced allosteric transitions in hemoglobin: the T-to-T(high) quaternary transitions.
Kavanaugh JS, Rogers PH, Arnone A
Biochemistry 44 6101-21 (2005)
PMID: 15835899

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 15.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand OXY 4 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 96.9Å b: 99.2Å c: 66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.176 0.212
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Hemoglobin: Structure, Function and Allostery.
Ahmed et al. (2020)
7 more

1 mention without citation

A New Look at the Structures of Old Sepsis Actors by Exploratory Data Analysis Tools.
Gnoni et al. (2019)