Function and Biology Details
Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
- not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138133 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Mdm2
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.17 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain
Length: 107 amino acids
Theoretical weight: 12.17 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
- Canonical:
P56273 (Residues: 13-119; Coverage: 23%)
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain
Cellular tumor antigen p53
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 2.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Sequence domains: P53 transactivation motif
Length: 17 amino acids
Theoretical weight: 2.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: P04637 (Residues: 13-29; Coverage: 4%)
Sequence domains: P53 transactivation motif
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Spacegroup:
P6322
Expression systems:
- Escherichia coli
- Not provided
