› › Structure analysis

Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60A mutant

X-ray diffraction
1.55Å resolution
PDB entry 1y34 coloured by chain, front view.
PDB entry 1y34 coloured by chain, side view.
PDB entry 1y34 coloured by chain, top view.
Source organisms:
Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents: 2 distinct polypeptide molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: Subtilisin BPN' and Chymotrypsin inhibitor 2
hide full assembly name
Multimeric state: hetero dimer
Accessible surface area: 13580.84 Å2
Buried surface area: 2422.74 Å2
Dissociation area: 800.63 Å2
Dissociation energy (ΔGdiss): 7.46 kcal/mol
Dissociation entropy (TΔSdiss): 10.76 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-133535
Assembly 2
Download    3D Visualisation
Assembly Name: Subtilisin BPN' and Chymotrypsin inhibitor 2
hide full assembly name
Multimeric state: hetero tetramer
Accessible surface area: 26166.1 Å2
Buried surface area: 5823.72 Å2
Dissociation area: 2,125.91 Å2
Dissociation energy (ΔGdiss): 1.3 kcal/mol
Dissociation entropy (TΔSdiss): 34.88 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-133538

Macromolecules

Subtilisin BPN'
Chain: E
Length: 281 amino acids
Theoretical weight: 28.38 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00782 (Residues: 108-382; Coverage: 78%)
Gene name: apr
Pfam: Subtilase family
InterPro:
CATH: Peptidase S8/S53 domain
SCOP: Subtilases
Subtilisin BPN' in PDB entry 1y34, assembly 1, front view.
Subtilisin BPN' in PDB entry 1y34, assembly 1, side view.
Subtilisin BPN' in PDB entry 1y34, assembly 1, top view.
Molecule details ›

Search similar proteins

Chymotrypsin inhibitor 2
Chain: I
Length: 64 amino acids
Theoretical weight: 7.23 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q40059 (Residues: 21-84; Coverage: 76%)
Gene name: Ica-2
Pfam: Potato inhibitor I family
InterPro:
CATH: Trypsin Inhibitor V, subunit A
SCOP: CI-2 family of serine protease inhibitors
Chymotrypsin inhibitor 2 in PDB entry 1y34, assembly 1, front view.
Chymotrypsin inhibitor 2 in PDB entry 1y34, assembly 1, side view.
Chymotrypsin inhibitor 2 in PDB entry 1y34, assembly 1, top view.
Molecule details ›

Search similar proteins

Quick links