› 1y0l

X-ray diffraction
2.5Å resolution

Catalytic elimination antibody 34E4 in complex with hapten

Released:
DOI: 10.2210/pdb1y0l/pdb
PDB entry 1y0l coloured by chain, front view.
PDB entry 1y0l coloured by chain, side view.
PDB entry 1y0l coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural origins of efficient proton abstraction from carbon by a catalytic antibody.
Debler EW, Ito S, Seebeck FP, Heine A, Hilvert D, Wilson IA
Proc Natl Acad Sci U S A 102 4984-9 (2005)
PMID: 15788533

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213116 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Catalytic Antibody Fab 34E4 Light chain Chains: A, C, E, L
Molecule details ›
Chains: A, C, E, L
Length: 216 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Catalytic Antibody Fab 34E4 Heavy chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 226 amino acids
Theoretical weight: 24.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand HAN 4 x HAN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3221
Unit cell:
a: 165.2Å b: 165.2Å c: 152.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.24
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Design of protein catalysts.
Hilvert D. (2013)
2 more

7 mentions without citation

Exploring challenges in rational enzyme design by simulating the catalysis in artificial kemp eliminase.
Frushicheva et al. (2010)
6 more