› 1xxw

X-ray diffraction
2.7Å resolution

Structure of zinc induced heterodimer of two calcium free isoforms of phospholipase A2 from Naja naja sagittifera at 2.7A resolution

Released:
DOI: 10.2210/pdb1xxw/pdb
PDB entry 1xxw coloured by chain, front view.
PDB entry 1xxw coloured by chain, side view.
PDB entry 1xxw coloured by chain, top view.
Source organism: Naja sagittifera
Primary publication:
Structure of the zinc-induced heterodimer of two calcium-free isoforms of phospholipase A2 from Naja naja sagittifera at 2.7 angstroms resolution.
Jabeen T, Sharma S, Singh N, Singh RK, Verma AK, Paramasivam M, Srinivasan A, Singh TP
Acta Crystallogr D Biol Crystallogr 61 302-8 (2005)
PMID: 15735340

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157962 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Basic phospholipase A2 1 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.22 KDa
Source organism: Naja sagittifera
UniProt:
  • Canonical: P60043 (Residues: 8-126; Coverage: 94%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain
Acidic phospholipase A2 2 Chain: B
Molecule details ›
Chain: B
Length: 119 amino acids
Theoretical weight: 13.29 KDa
Source organism: Naja sagittifera
UniProt:
  • Canonical: P60044 (Residues: 8-126; Coverage: 94%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41
Unit cell:
a: 65.467Å b: 65.467Å c: 58.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.188 0.232

Quick links

Citations

1 citations in other articles

Crystal structure of a heterodimer of phospholipase A2 from Naja naja sagittifera at 2.3 A resolution reveals the presence of a new PLA2-like protein with a novel cys 32-Cys 49 disulphide bridge with a bound sugar at the substrate-binding site.
Jabeen et al. (2006)

2 mentions without citation

Electrostatic properties of protein-protein complexes.
Kundrotas et al. (2006)
1 more