X-ray diffraction
2.4Å resolution

Crystal Structure of CaiB mutant D169A in complex with carnitinyl-CoA


Function and Biology Details

Reaction catalysed:
(E)-4-(trimethylammonio)but-2-enoyl-CoA + L-carnitine = (E)-4-(trimethylammonio)but-2-enoate + L-carnitinyl-CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
L-carnitine CoA-transferase Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 45.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
  • Canonical: P31572 (Residues: 1-405; Coverage: 100%)
Gene names: JW0037, b0038, caiB, yaaN
Sequence domains: CoA-transferase family III
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 86.627Å b: 86.627Å c: 163.863Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.205 0.252
Expression system: Escherichia coli BL21