1xpo

X-ray diffraction
3.15Å resolution

Structural mechanism of inhibition of the Rho transcription termination factor by the antibiotic bicyclomycin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription termination factor Rho Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 419 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG30 (Residues: 1-419; Coverage: 100%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
Structure domains:
5'-R(*CP*UP*CP*UP*CP*UP*CP*U)-3' Chains: G, H, J, K, L, M
Molecule details ›
Chains: G, H, J, K, L, M
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 211.933Å b: 111.37Å c: 161.033Å
α: 90° β: 108.79° γ: 90°
R-values:
R R work R free
0.29 0.285 0.306
Expression systems:
  • Escherichia coli
  • Not provided