› 1xj2

X-ray diffraction
2Å resolution

CO-bound structure of bjFixLH

Released:
DOI: 10.2210/pdb1xj2/pdb
PDB entry 1xj2 coloured by chain, front view.
PDB entry 1xj2 coloured by chain, side view.
PDB entry 1xj2 coloured by chain, top view.
Source organism: Bradyrhizobium japonicum
Primary publication:
Crystal structures of deoxy and CO-bound bjFixLH reveal details of ligand recognition and signaling.
Key J, Moffat K
Biochemistry 44 4627-35 (2005)
PMID: 15779889

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor protein FixL Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.1 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P23222 (Residues: 154-269; Coverage: 23%)
Gene names: bll2760, fixL
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand CMO 1 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: R32
Unit cell:
a: 128.458Å b: 128.458Å c: 58.536Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.256
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
5 more

3 mentions without citation

The enigmatic cytoplasmic regions of KCNH channels.
Morais-Cabral et al. (2015)
2 more