Structure analysis

HincII bound to cleaved, cognate DNA containing GTCGAC

X-ray diffraction
2.95Å resolution
Source organism: Haemophilus influenzae
Assembly composition:
hetero hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 22900 Å2
Buried surface area: 11500 Å2
Dissociation area: 1,000 Å2
Dissociation energy (ΔGdiss): 13 kcal/mol
Dissociation entropy (TΔSdiss): 9 kcal/mol
Interface energy (ΔGint): -66 kcal/mol
Symmetry number: 1
Assembly 2
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Multimeric state: hetero hexamer

Binding statistics and energies are not available for this assembly

Macromolecules

Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 29.82 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Pfam: Restriction endonuclease HincII
InterPro:
CATH: DNA mismatch repair MutH/Restriction endonuclease, type II
SCOP: Restriction endonuclease HincII

Search similar proteins

Name: 5'-D(*GP*CP*CP*GP*GP*TP*C)-3'
Representative chains: E, G, I, K
Source organism: Haemophilus influenzae [727]
Expression system: Not provided
Length: 7 nucleotides
Theoretical weight: 2.11 KDa

Search similar DNA

Name: 5'-D(P*GP*AP*CP*CP*GP*G)-3'
Representative chains: F, H, J, L
Source organism: Haemophilus influenzae [727]
Expression system: Not provided
Length: 6 nucleotides
Theoretical weight: 1.83 KDa

Search similar DNA