X-ray diffraction
1.8Å resolution

1.8A crystal structure of formiminoglutamase from Vibrio cholerae O1 biovar eltor str. N16961

Source organism: Vibrio cholerae
Entry authors: Wu R, Zhang R, Shonda C, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
N-formimidoyl-L-glutamate + H(2)O = L-glutamate + formamide
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Formimidoylglutamase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 37.37 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
  • Canonical: Q9KSQ2 (Residues: 1-336; Coverage: 100%)
Gene names: VC_1204, hutG
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 139.278Å b: 139.278Å c: 134.488Å
α: 90° β: 90° γ: 120°
R R work R free
0.202 0.202 0.229
Expression system: Escherichia coli BL21