1xdb

X-ray diffraction
2.8Å resolution

Crystal Structure of the Nitrogenase Fe protein Asp129Glu

Released:

Function and Biology Details

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-132539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogenase iron protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 31.43 KDa
Source organism: Azotobacter vinelandii
Expression system: Escherichia coli
UniProt:
  • Canonical: P00459 (Residues: 2-290; Coverage: 100%)
Gene name: nifH1
Sequence domains: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 57.574Å b: 92.138Å c: 63.934Å
α: 90° β: 100.799° γ: 90°
R-values:
R R work R free
0.305 0.227 0.299
Expression system: Escherichia coli