1x8e

X-ray diffraction
2.8Å resolution

Crystal structure of Pyrococcus furiosus phosphoglucose isomerase free enzyme

Released:

Function and Biology Details

Reaction catalysed:
D-glucose 6-phosphate = D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.64 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83194 (Residues: 1-189; Coverage: 100%)
Gene names: PF0196, pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 68.236Å b: 73.865Å c: 77.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.276
Expression system: Escherichia coli BL21(DE3)