PDBe 1x86

X-ray diffraction
3.22Å resolution

Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF in complex with RhoA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 12 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 385 amino acids
Theoretical weight: 44.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZN5 (Residues: 766-1138; Coverage: 24%)
Gene names: ARHGEF12, KIAA0382, LARG
Sequence domains:
Structure domains:
Transforming protein RhoA Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 196 amino acids
Theoretical weight: 22.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 1-193; Coverage: 100%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 296.418Å b: 95.239Å c: 157.338Å
α: 90° β: 94.19° γ: 90°
R-values:
R R work R free
0.23 0.227 0.279
Expression system: Escherichia coli