› 1wwj

X-ray diffraction
1.9Å resolution

crystal structure of KaiB from Synechocystis sp.

Released:
DOI: 10.2210/pdb1wwj/pdb
PDB entry 1wwj coloured by chain, front view.
PDB entry 1wwj coloured by chain, side view.
PDB entry 1wwj coloured by chain, top view.
Source organism: Synechocystis sp.
Primary publication:
Tetrameric architecture of the circadian clock protein KaiB. A novel interface for intermolecular interactions and its impact on the circadian rhythm.
Hitomi K, Oyama T, Han S, Arvai AS, Getzoff ED
J Biol Chem 280 19127-35 (2005)
PMID: 15716274

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiB1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.95 KDa
Source organism: Synechocystis sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P74645 (Residues: 1-105; Coverage: 100%)
Gene names: kaiB1, slr0757
Sequence domains: KaiB domain
Structure domains: Glutaredoxin

Ligands and Environments

3 bound ligands:
Ligand MLT 8 x MLT
Ligand BET 5 x BET
Ligand IMD 3 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 52.77Å b: 66.036Å c: 114.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.284
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Circadian Rhythms in Cyanobacteria.
Cohen et al. (2015)
12 more

4 mentions without citation

A cyanobacterial circadian clockwork.
Johnson et al. (2008)
3 more