› 1wuu

X-ray diffraction
2.5Å resolution

crystal structure of human galactokinase complexed with MgAMPPNP and galactose

Released:
DOI: 10.2210/pdb1wuu/pdb
PDB entry 1wuu coloured by chain, front view.
PDB entry 1wuu coloured by chain, side view.
PDB entry 1wuu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular structure of human galactokinase: implications for type II galactosemia.
Thoden JB, Timson DJ, Reece RJ, Holden HM
J Biol Chem 280 9662-70 (2005)
PMID: 15590630

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactokinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 399 amino acids
Theoretical weight: 43.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51570 (Residues: 2-392; Coverage: 100%)
Gene names: GALK, GALK1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLA 4 x GLA
Ligand MG 4 x MG
Ligand ANP 4 x ANP
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P21
Unit cell:
a: 73.2Å b: 109.6Å c: 115.8Å
α: 90° β: 95.9° γ: 90°
R-values:
R R work R free
0.205 0.201 0.258
Expression system: Escherichia coli

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Citations

6 review citations

Enzymatic methods for glyco(diversification/randomization) of drugs and small molecules.
Gantt et al. (2011)
5 more

11 mentions without citation

The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Lavy et al. (2012)
10 more