1wq1

X-ray diffraction
2.5Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ras GTPase-activating protein 1 Chain: G
Molecule details ›
Chain: G
Length: 334 amino acids
Theoretical weight: 38.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20936 (Residues: 714-1047; Coverage: 32%)
Gene names: GAP, RASA, RASA1
Sequence domains: GTPase-activator protein for Ras-like GTPase
Structure domains: GTPase Activation - p120gap; domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-18
Spacegroup: P21
Unit cell:
a: 71.9Å b: 41.1Å c: 89Å
α: 90° β: 108.4° γ: 90°
R-values:
R R work R free
0.233 0.233 0.319
Expression system: Escherichia coli