PDBe 1wl4

X-ray diffraction
1.55Å resolution

Human cytosolic acetoacetyl-CoA thiolase complexed with CoA

Released:

Function and Biology Details

Reaction catalysed:
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase, cytosolic Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 41.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BWD1 (Residues: 1-397; Coverage: 100%)
Gene names: ACAT2, ACTL
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COA 1 x COA
2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 57.86Å b: 109.83Å c: 155.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.107 0.106 0.14
Expression system: Escherichia coli