1wkw

X-ray diffraction
2.1Å resolution

Crystal structure of the ternary complex of eIF4E-m7GpppA-4EBP1 peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06730 (Residues: 27-217; Coverage: 88%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E-binding protein 1 Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13541 (Residues: 47-66; Coverage: 17%)
Gene name: EIF4EBP1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P43
Unit cell:
a: 87.77Å b: 87.77Å c: 38.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.27
Expression system: Escherichia coli