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Trimeric Structure of the Enzyme IIA from Escherichia coli Phosphotransferase System Specific for N,N'-Diacetylchitobiose

Solution NMR
PDB entry 1wcr coloured by chain, front view.
PDB entry 1wcr coloured by chain, side view.
PDB entry 1wcr coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
homo trimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: PTS system N,N'-diacetylchitobiose-specific EIIA component
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Multimeric state: homo trimer
Accessible surface area: 16075.09 Å2
Buried surface area: 5444.41 Å2
Dissociation area: 2,722.21 Å2
Dissociation energy (ΔGdiss): 20.9 kcal/mol
Dissociation entropy (TΔSdiss): 23.83 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-159662

Macromolecules

PTS system N,N'-diacetylchitobiose-specific EIIA component
Chains: A, B, C
Length: 103 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69791 (Residues: 14-116; Coverage: 89%)
Gene names: JW1725, b1736, celC, chbA
Pfam: PTS system, Lactose/Cellobiose specific IIA subunit
InterPro:
CATH: Phosphotransferase system, lactose/cellobiose-type IIA subunit
PTS system N,N'-diacetylchitobiose-specific EIIA component in PDB entry 1wcr, assembly 1, front view.
PTS system N,N'-diacetylchitobiose-specific EIIA component in PDB entry 1wcr, assembly 1, side view.
PTS system N,N'-diacetylchitobiose-specific EIIA component in PDB entry 1wcr, assembly 1, top view.
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