› 1w8d

X-ray diffraction
2.2Å resolution

Binary structure of human DECR.

Released:
DOI: 10.2210/pdb1w8d/pdb
PDB 1w8d coloured by chain and viewed from the front.
PDB 1w8d coloured by chain and viewed from the side.
PDB 1w8d coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structure and reactivity of human mitochondrial 2,4-dienoyl-CoA reductase: enzyme-ligand interactions in a distinctive short-chain reductase active site.
Alphey MS, Yu W, Byres E, Li D, Hunter WN
J. Biol. Chem. 280 3068-77 (2005)
PMID: 15531764

Function and Biology Details

Reaction catalysed: 
A (2E)-2-enoyl-CoA + NADP(+) = a (2E,4E)-2,4-dienoyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 302 amino acids
Theoretical weight: 32.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q16698 (Residues: 34-335; Coverage: 90%)
Gene names: DECR, DECR1, SDR18C1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 62.873Å b: 131.702Å c: 70.592Å
α: 90° β: 92.56° γ: 90°
R-values:
R R work R free
0.221 0.219 0.265
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Order, Disorder, and Everything in Between.
DeForte et al. (2016)
2 more