› 1w0u

X-ray diffraction
1.8Å resolution

hTRF2 DNA-binding domain in complex with telomeric DNA.

Released:
DOI: 10.2210/pdb1w0u/pdb
PDB entry 1w0u coloured by chain, front view.
PDB entry 1w0u coloured by chain, side view.
PDB entry 1w0u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
How the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA: a view from high-resolution crystal structures.
Court R, Chapman L, Fairall L, Rhodes D
EMBO Rep 6 39-45 (2005)
PMID: 15608617

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114756 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Telomeric repeat-binding factor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 55 amino acids
Theoretical weight: 6.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15554 (Residues: 488-542; Coverage: 10%)
Gene names: TERF2, TRBF2, TRF2
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
5'-D(*TP*CP*TP*AP*GP*GP*GP*TP*TP*AP *GP*GP*GP*TP*TP*AP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP *CP*CP*CP*TP*AP*GP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.1 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 44.08Å b: 46.78Å c: 107.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.242
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

How shelterin protects mammalian telomeres.
Palm et al. (2008)
40 more

13 mentions without citation

Finding the end: recruitment of telomerase to telomeres.
Nandakumar et al. (2013)
12 more