› 1vra

X-ray diffraction
2Å resolution

Crystal structure of Arginine biosynthesis bifunctional protein argJ (10175521) from Bacillus halodurans at 2.00 A resolution

Released:
DOI: 10.2210/pdb1vra/pdb
PDB 1vra coloured by chain and viewed from the front.
PDB 1vra coloured by chain and viewed from the side.
PDB 1vra coloured by chain and viewed from the top.
Source organism: Halalkalibacterium halodurans
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Arginine biosynthesis bifunctional protein ArgJ alpha chain Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.35 KDa
Source organism: Halalkalibacterium halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K8V3 (Residues: 1-196; Coverage: 48%)
Gene names: BH2899, argJ
Sequence domains: ArgJ family
Structure domains: L-amino peptidase D-ALA esterase/amidase
Arginine biosynthesis bifunctional protein ArgJ beta chain Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 23.2 KDa
Source organism: Halalkalibacterium halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K8V3 (Residues: 197-411; Coverage: 52%)
Gene names: BH2899, argJ
Sequence domains: ArgJ family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand UNL 1 x UNL
Ligand EDO 16 x EDO
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 70.658Å b: 70.658Å c: 222.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.158
Expression system: Escherichia coli

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