PDBe 1vol

X-ray diffraction
2.7Å resolution

TFIIB (HUMAN CORE DOMAIN)/TBP (A.THALIANA)/TATA ELEMENT TERNARY COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription initiation factor IIB Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00403 (Residues: 113-316; Coverage: 65%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains: Transcription factor TFIIB repeat
Structure domains: Cyclin-like
TATA-box-binding protein 1 Chain: B
Molecule details ›
Chain: B
Length: 200 amino acids
Theoretical weight: 22.4 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P28147 (Residues: 1-200; Coverage: 100%)
Gene names: At3g13445, MRP15.10, TBP1
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
DNA (5'-D(*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
DNA (5'-D(*CP*AP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.81 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 69.9Å b: 78Å c: 134.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.318
Expression system: Escherichia coli