› 1vol

X-ray diffraction
2.7Å resolution

TFIIB (HUMAN CORE DOMAIN)/TBP (A.THALIANA)/TATA ELEMENT TERNARY COMPLEX

Released:
DOI: 10.2210/pdb1vol/pdb
PDB 1vol coloured by chain and viewed from the front.
PDB 1vol coloured by chain and viewed from the side.
PDB 1vol coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Crystal structure of a TFIIB-TBP-TATA-element ternary complex.
Nikolov DB, Chen H, Halay ED, Usheva AA, Hisatake K, Lee DK, Roeder RG, Burley SK
Nature 377 119-28 (1995)
PMID: 7675079

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription initiation factor IIB Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00403 (Residues: 113-316; Coverage: 65%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains: Transcription factor TFIIB repeat
Structure domains: Cyclin-like
TATA-box-binding protein 1 Chain: B
Molecule details ›
Chain: B
Length: 200 amino acids
Theoretical weight: 22.4 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P28147 (Residues: 1-200; Coverage: 100%)
Gene names: At3g13445, MRP15.10, TBP1
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
DNA (5'-D(*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
DNA (5'-D(*CP*AP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.81 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 69.9Å b: 78Å c: 134.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.318
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Beyond the canonical role of TFIIB in eukaryotic transcription.
O'Brien et al. (2022)
67 more

19 mentions without citation

Energy Storage Application of All-Organic Polymer Dielectrics: A Review.
Yang et al. (2022)
18 more