› 1vlo

X-ray diffraction
1.7Å resolution

Crystal structure of aminomethyltransferase (T protein; tetrahydrofolate-dependent) of glycine cleavage system (np417381) from Escherichia coli k12 at 1.70 A resolution

Released:
DOI: 10.2210/pdb1vlo/pdb
PDB 1vlo coloured by chain and viewed from the front.
PDB 1vlo coloured by chain and viewed from the side.
PDB 1vlo coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminomethyltransferase Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 42.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P27248 (Residues: 2-364; Coverage: 100%)
Gene names: JW2873, b2905, gcvT
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 46.517Å b: 64.564Å c: 117.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.195
Expression system: Escherichia coli

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