› 1vfy

X-ray diffraction
1.15Å resolution

PHOSPHATIDYLINOSITOL-3-PHOSPHATE BINDING FYVE DOMAIN OF VPS27P PROTEIN FROM SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1vfy/pdb
PDB 1vfy coloured by chain and viewed from the front.
PDB 1vfy coloured by chain and viewed from the side.
PDB 1vfy coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a phosphatidylinositol 3-phosphate-specific membrane-targeting motif, the FYVE domain of Vps27p.
Misra S, Hurley JH
Cell 97 657-66 (1999)
PMID: 10367894

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 27 Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40343 (Residues: 163-231; Coverage: 11%)
Gene names: DID7, GRD11, N2038, SSV17, VPL23, VPS27, VPT27, YNR006W
Sequence domains: FYVE zinc finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P1
Unit cell:
a: 24.09Å b: 26.57Å c: 31.61Å
α: 111.79° β: 92.7° γ: 105.7°
R-values:
R R work R free
0.174 0.174 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

48 review citations

Regulation of V-ATPase Activity and Organelle pH by Phosphatidylinositol Phosphate Lipids.
Banerjee et al. (2020)
47 more

22 mentions without citation

Cellular and molecular interactions of phosphoinositides and peripheral proteins.
Stahelin et al. (2014)
21 more