X-ray diffraction
2.21Å resolution

X-ray crystal structure of the Tryptophan Synthase b2 Subunit from Hyperthermophile, Pyrococcus furiosus


Function and Biology Details

Reaction catalysed:
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Tryptophan synthase beta chain 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 42.59 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
  • Canonical: Q8U093 (Residues: 1-388; Coverage: 100%)
Gene names: PF1706, trpB1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

Cofactor: Ligand PLP 4 x PLP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 84.835Å b: 110.53Å c: 159.999Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.208 0.263
Expression system: Escherichia coli