1v5z

X-ray diffraction
2Å resolution

Binding of coumarins to NAD(P)H:FMN oxidoreductase

Released:
Source organism: Aliivibrio fischeri
Entry authors: Kobori T, Koike H, Sasaki H, Zenno S, Saigo K, Tanokura M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major NAD(P)H-flavin oxidoreductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 24.62 KDa
Source organism: Aliivibrio fischeri
Expression system: Escherichia coli
UniProt:
  • Canonical: P46072 (Residues: 2-218; Coverage: 100%)
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 101.6Å b: 63.3Å c: 74.4Å
α: 90° β: 100° γ: 90°
R-values:
R R work R free
0.201 0.201 0.248
Expression system: Escherichia coli