› 1v5x

X-ray diffraction
2Å resolution

Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus

Released:
DOI: 10.2210/pdb1v5x/pdb
PDB entry 1v5x coloured by chain, front view.
PDB entry 1v5x coloured by chain, side view.
PDB entry 1v5x coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments.
Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, Yokoyama S, Yutani K
J Biochem 137 569-78 (2005)
PMID: 15944409

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-(5'-phosphoribosyl)anthranilate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 21.98 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83825 (Residues: 1-203; Coverage: 100%)
Gene names: TthHB5018_03080, trpF
Sequence domains: N-(5'phosphoribosyl)anthranilate (PRA) isomerase
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P4132
Unit cell:
a: 144.205Å b: 144.205Å c: 144.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.262 0.296
Expression system: Escherichia coli

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Citations

3 citation in other articles

Metal ion binding to anticoagulation factor II from the venom of Agkistrodon acutus: stabilization of the structure and regulation of the binding affinity to activated coagulation factor X.
Shen et al. (2011)
2 more

2 mentions without citation

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
1 more