1v4n

X-ray diffraction
2.45Å resolution

Structure of 5'-deoxy-5'-methylthioadenosine phosphorylase homologue from Sulfolobus tokodaii

Released:
Source organism: Sulfurisphaera tokodaii
Entry authors: Kitago Y, Yasutake Y, Sakai N, Tsujimura M, Yao M, Watanabe N, Kawarabayasi Y, Tanaka I

Function and Biology Details

Reaction catalysed:
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-methyl-5'-thioadenosine phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 281 amino acids
Theoretical weight: 31.58 KDa
Source organism: Sulfurisphaera tokodaii
Expression system: Escherichia coli
UniProt:
  • Canonical: F9VN03 (Residues: 1-270; Coverage: 100%)
Gene names: ST0485, STK_04850, mtaP, mtnP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: C2221
Unit cell:
a: 79.869Å b: 142.888Å c: 169.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.241
Expression system: Escherichia coli