› 1v47

X-ray diffraction
2.49Å resolution

Crystal structure of ATP sulfurylase from Thermus thermophillus HB8 in complex with APS

Released:
DOI: 10.2210/pdb1v47/pdb
PDB entry 1v47 coloured by chain, front view.
PDB entry 1v47 coloured by chain, side view.
PDB entry 1v47 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of a novel zinc-binding ATP sulfurylase from Thermus thermophilus HB8.
Taguchi Y, Sugishima M, Fukuyama K
Biochemistry 43 4111-8 (2004)
PMID: 15065853

Function and Biology Details

Reaction catalysed: 
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
sulfate adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 38.9 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKH7 (Residues: 1-349; Coverage: 100%)
Gene name: TTHA0666
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 5 x CL
Ligand NA 4 x NA
Ligand ADX 2 x ADX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21
Unit cell:
a: 68.858Å b: 61.254Å c: 128.665Å
α: 90° β: 95.43° γ: 90°
R-values:
R R work R free
0.226 0.219 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Diversity and regulation of ATP sulfurylase in photosynthetic organisms.
Prioretti et al. (2014)
3 more

3 mentions without citation

Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus.
Jespersen et al. (2023)
2 more