› 1uww

X-ray diffraction
1.4Å resolution

X-ray crystal structure of a non-crystalline cellulose specific carbohydrate-binding module: CBM28.

Released:
DOI: 10.2210/pdb1uww/pdb
PDB entry 1uww coloured by chain, front view.
PDB entry 1uww coloured by chain, side view.
PDB entry 1uww coloured by chain, top view.
Source organism: Alkalihalobacillus akibai
Primary publication:
X-ray crystal structure of a non-crystalline cellulose-specific carbohydrate-binding module: CBM28.
Jamal S, Nurizzo D, Boraston AB, Davies GJ
J Mol Biol 339 253-8 (2004)
PMID: 15136030

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.75 KDa
Source organism: Alkalihalobacillus akibai
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06564 (Residues: 571-761; Coverage: 25%)
Structure domains: Galactose-binding domain-like

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 63.866Å b: 65.926Å c: 103.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Recombinant CBM-fusion technology - Applications overview.
Oliveira et al. (2015)
2 more

1 mention without citation

Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin.
Georgelis et al. (2012)