› 1uw0

Solution NMR

Solution structure of the zinc-finger domain from DNA ligase IIIa

Released:
DOI: 10.2210/pdb1uw0/pdb
PDB entry 1uw0 coloured by chain, ensemble of 28 models, front view.
PDB entry 1uw0 coloured by chain, ensemble of 28 models, side view.
PDB entry 1uw0 coloured by chain, ensemble of 28 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIalpha.
Kulczyk AW, Yang JC, Neuhaus D
J Mol Biol 341 723-38 (2004)
PMID: 15288782

Function and Biology Details

Reaction catalysed: 
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase 3 Chain: A
Molecule details ›
Chain: A
Length: 117 amino acids
Theoretical weight: 13.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49916 (Residues: 88-204; Coverage: 12%)
Gene name: LIG3
Sequence domains: Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
Structure domains: Zinc finger, PARP-type

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review).
Bertin et al. (2006)
4 more

6 mentions without citation

The DNA-binding domain of human PARP-1 interacts with DNA single-strand breaks as a monomer through its second zinc finger.
Eustermann et al. (2011)
5 more