1ut9

X-ray diffraction
2.1Å resolution

Structural Basis for the Exocellulase Activity of the Cellobiohydrolase CbhA from C. thermocellum

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 609 amino acids
Theoretical weight: 68.61 KDa
Source organism: Hungateiclostridium thermocellum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6RSN8 (Residues: 2-607; Coverage: 100%)
Gene name: cbhA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P212121
Unit cell:
a: 74.546Å b: 75.76Å c: 137.498Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.186
Expression system: Escherichia coli BL21(DE3)