1usm

X-ray diffraction
1.2Å resolution

DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR, PRO9LEU MUTANT

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Tahirov TH, Inagaki E

Function and Biology Details

Reaction catalysed:
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4a-hydroxytetrahydrobiopterin dehydratase Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 9.51 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83819 (Residues: 1-80; Coverage: 100%)
Sequence domains: Pterin 4 alpha carbinolamine dehydratase
Structure domains: Transcriptional coactivator/pterin dehydratase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I422
Unit cell:
a: 55.625Å b: 55.625Å c: 125.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.228
Expression system: Escherichia coli BL21(DE3)