1usk

X-ray diffraction
2Å resolution

L-leucine-binding protein with leucine bound

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-specific-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 346 amino acids
Theoretical weight: 37.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04816 (Residues: 24-369; Coverage: 100%)
Gene names: JW3423, b3458, livK
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P1
Unit cell:
a: 68.421Å b: 70.14Å c: 79.902Å
α: 112.79° β: 93.23° γ: 90.97°
R-values:
R R work R free
0.214 0.212 0.252
Expression system: Escherichia coli BL21