› 1unu

X-ray diffraction
2.07Å resolution

Structure Based Engineering of Internal Molecular Surfaces Of Four Helix Bundles

Released:
DOI: 10.2210/pdb1unu/pdb
PDB 1unu coloured by chain and viewed from the front.
PDB 1unu coloured by chain and viewed from the side.
PDB 1unu coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure-based engineering of internal cavities in coiled-coil peptides.
Yadav MK, Redman JE, Leman LJ, Alvarez-Gutiérrez JM, Zhang Y, Stout CD, Ghadiri MR
Biochemistry 44 9723-32 (2005)
PMID: 16008357

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 34 amino acids
Theoretical weight: 4.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4132
Unit cell:
a: 78.486Å b: 78.486Å c: 78.486Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.273
Expression system: Not provided

Quick links

Citations

2 review citations

Design of catalytic polypeptides and proteins.
Gutte et al. (2018)
1 more

1 mention without citation

Structure-based engineering of internal cavities in coiled-coil peptides.
Yadav et al. (2005)