› 1unj

X-ray diffraction
2.5Å resolution

Crystal structure of a 7-Aminoactinomycin D complex with non-complementary DNA

Released:
DOI: 10.2210/pdb1unj/pdb
PDB entry 1unj coloured by chain, front view.
PDB entry 1unj coloured by chain, side view.
PDB entry 1unj coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT).
Alexopoulos E, Jares-Erijman EA, Jovin TM, Klement R, Machinek R, Sheldrick GM, Usón I
Acta Crystallogr D Biol Crystallogr 61 407-15 (2005)
PMID: 15805595

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-114702 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
7-AMINO-ACTINOMYCIN D Chains: E, F, K, L, Q, R, W, X
Molecule details ›
Chains: E, F, K, L, Q, R, W, X
Length: 11 amino acids
Theoretical weight: 1.27 KDa
Source organism: Streptomyces antibioticus
Expression system: Not provided
5'-D(*TP*TP*AP*GP*BRU*TP)-3' Chains: A, B, C, D, G, H, I, J, M, N, O, P, S, T, U, V
Molecule details ›
Chains: A, B, C, D, G, H, I, J, M, N, O, P, S, T, U, V
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
Source organism: Streptomyces antibioticus
Expression system: Not provided

Ligands and Environments

No bound ligands
4 modified residues:
Ligand BRU 16 x BRU
Ligand DVA 16 x DVA
Ligand SAR 16 x SAR
Ligand MVA 16 x MVA

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P3221
Unit cell:
a: 71.222Å b: 71.222Å c: 108.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.276 not available 0.316
Expression system: Not provided

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Citations

4 citation in other articles

Force spectroscopy reveals the DNA structural dynamics that govern the slow binding of Actinomycin D.
Paramanathan et al. (2012)
3 more