› 1umz

X-ray diffraction
1.8Å resolution

Xyloglucan endotransglycosylase in complex with the xyloglucan nonasaccharide XLLG.

Released:
DOI: 10.2210/pdb1umz/pdb
PDB 1umz coloured by chain and viewed from the front.
PDB 1umz coloured by chain and viewed from the side.
PDB 1umz coloured by chain and viewed from the top.
Source organism: Populus tremula
Primary publication:
Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding.
Johansson P, Brumer H, Baumann MJ, Kallas AM, Henriksson H, Denman SE, Teeri TT, Jones TA
Plant Cell 16 874-86 (2004)
PMID: 15020748

Function and Biology Details

Reaction catalysed: 
Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Xyloglucan endotransglucosylase protein 34 Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 32.53 KDa
Source organism: Populus tremula
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q8GZD5 (Residues: 23-294; Coverage: 100%)
Gene names: XET16A, XTH16-34
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, XYS, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P63
Unit cell:
a: 188.162Å b: 188.162Å c: 45.896Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.222
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Biochemistry and physiological roles of enzymes that 'cut and paste' plant cell-wall polysaccharides.
Franková et al. (2013)
4 more

7 mentions without citation

Molecular modeling of family GH16 glycoside hydrolases: potential roles for xyloglucan transglucosylases/hydrolases in cell wall modification in the poaceae.
Strohmeier et al. (2004)
6 more