PDBe 1ul1

X-ray diffraction
2.9Å resolution

Crystal structure of the human FEN1-PCNA complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Flap endonuclease 1 Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 379 amino acids
Theoretical weight: 42.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39748 (Residues: 2-380; Coverage: 100%)
Gene names: FEN1, RAD2
Sequence domains: XPG I-region
Structure domains: 5'-nuclease
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 82.203Å b: 143.383Å c: 246.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.284
Expression system: Escherichia coli BL21(DE3)