1ukl

X-ray diffraction
3Å resolution

Crystal structure of Importin-beta and SREBP-2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit beta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 876 amino acids
Theoretical weight: 97.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P70168 (Residues: 1-876; Coverage: 100%)
Gene names: Impnb, Kpnb1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Processed sterol regulatory element-binding protein 2 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 61 amino acids
Theoretical weight: 7.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12772 (Residues: 343-403; Coverage: 5%)
Gene names: BHLHD2, SREBF2, SREBP2
Structure domains: Helix-loop-helix DNA-binding domain

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 101.092Å b: 113.285Å c: 240.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.297
Expression system: Escherichia coli BL21