PDBe 1ujk

X-ray diffraction
1.9Å resolution

VHS domain of human GGA1 complexed with C-terminal phosphopeptide from BACE

Released:

Function and Biology Details

Reaction catalysed:
Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor-binding protein GGA1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UJY5 (Residues: 1-147; Coverage: 23%)
Gene name: GGA1
Sequence domains: VHS domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Beta-secretase 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P56817 (Residues: 490-501; Coverage: 3%)
Gene names: BACE, BACE1, KIAA1149

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 48.545Å b: 72.308Å c: 103.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.245
Expression systems:
  • Escherichia coli BL21
  • Not provided