X-ray diffraction
0.99Å resolution

Structure of beta-glucosidase at atomic resolution from thermus thermophilus HB8

Source organism: Thermus thermophilus
Entry authors: Lokanath NK, Shiromizu I, Miyano M, Yokoyama S, Kuramitsu S, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 48.7 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q8GEB3 (Residues: 1-431; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 109.092Å b: 116.463Å c: 87.362Å
α: 90° β: 90° γ: 90°
R R work R free
0.122 0.121 0.13
Expression system: Escherichia coli BL21(DE3)