1uex

X-ray diffraction
2.85Å resolution

Crystal structure of von Willebrand Factor A1 domain complexed with snake venom bitiscetin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Snaclec bitiscetin subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.95 KDa
Source organism: Bitis arietans
UniProt:
  • Canonical: Q7LZK5 (Residues: 1-131; Coverage: 100%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Snaclec bitiscetin subunit beta Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 14.82 KDa
Source organism: Bitis arietans
UniProt:
  • Canonical: Q7LZK8 (Residues: 1-125; Coverage: 100%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
von Willebrand factor Chain: C
Molecule details ›
Chain: C
Length: 209 amino acids
Theoretical weight: 23.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04275 (Residues: 1260-1468; Coverage: 8%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P43
Unit cell:
a: 89.282Å b: 89.282Å c: 53.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.276
Expression system: Escherichia coli BL21(DE3)