› 1ue1

X-ray diffraction
2.5Å resolution

Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1ue1/pdb
PDB entry 1ue1 coloured by chain, front view.
PDB entry 1ue1 coloured by chain, side view.
PDB entry 1ue1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications.
Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M
J Mol Biol 331 385-93 (2003)
PMID: 12888346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 17.37 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGD5 (Residues: 1-164; Coverage: 100%)
Gene names: MTCY21D4.17, Rv0054, ssb
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 78.72Å b: 78.72Å c: 77.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.288
Expression system: Escherichia coli

Quick links

Citations

7 review citations

From RPA to BRCA2: lessons from single-stranded DNA binding by the OB-fold.
Bochkarev et al. (2004)
6 more

3 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
2 more