X-ray diffraction
2.83Å resolution

Crystal structure of Human Phosphodiesterase 5 complexed with tadalafil(Cialis)


Function and Biology Details

Reaction catalysed:
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
cGMP-specific 3',5'-cyclic phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O76074 (Residues: 537-860; Coverage: 37%)
Gene names: PDE5, PDE5A
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: C2
Unit cell:
a: 131.352Å b: 48.561Å c: 123.863Å
α: 90° β: 117.28° γ: 90°
R R work R free
0.291 0.263 0.374
Expression system: Escherichia coli