1uay

X-ray diffraction
1.4Å resolution

Crystal Structure of Type II 3-Hydroxyacyl-CoA Dehydrogenase from Thermus thermophilus HB8

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Kunishima N, Asada Y, Yokoyama S, Kuramitsu S, Miyano M, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidoreductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 25.63 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SIA1 (Residues: 1-242; Coverage: 100%)
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 78.93Å b: 94.871Å c: 113.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.197
Expression system: Escherichia coli