› 1uax

X-ray diffraction
2Å resolution

Crystal structure of the ribonuclease H2 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1uax/pdb
PDB entry 1uax coloured by chain, front view.
PDB entry 1uax coloured by chain, side view.
PDB entry 1uax coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Hata T, Numata T, Kakuta Y, Kimura M

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HII Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 25.33 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59351 (Residues: 1-220; Coverage: 100%)
Gene names: PH1650, rnhB
Sequence domains: Ribonuclease HII
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 27.72Å b: 97.65Å c: 159.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.298
Expression system: Escherichia coli

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