› 1ua7

X-ray diffraction
2.21Å resolution

Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose

Released:
DOI: 10.2210/pdb1ua7/pdb
PDB 1ua7 coloured by chain and viewed from the front.
PDB 1ua7 coloured by chain and viewed from the side.
PDB 1ua7 coloured by chain and viewed from the top.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
Kagawa M, Fujimoto Z, Momma M, Takase K, Mizuno H
J. Bacteriol. 185 6981-4 (2003)
PMID: 14617662

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 46.8 KDa
Source organism: Bacillus subtilis
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00691 (Residues: 45-466; Coverage: 67%)
Gene names: BSU03040, amyA, amyE
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GLD
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, G6D
Carbohydrate polymer
2 bound ligands:
Ligand ACI 2 x ACI
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P212121
Unit cell:
a: 70.32Å b: 74.204Å c: 115.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.265
Expression system: Bacillus subtilis

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Citations

9 citation in other articles

Analysis of the key active subsites of glycoside hydrolase 13 family members.
Kumar V. (2010)
8 more

2 mentions without citation

Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
Kagawa et al. (2003)
1 more